Evaluation of drug-free methods for the detection of gene silencing in Saccharomyces cerevisiae

Autor:	Kholoud Shaban, Safia Mahabub Sauty, Ashley Fisher, Ashley Cheng, Krassimir Yankulov
Rok vydání:	2023
Předmět:	Cell Biology Molecular Biology Biochemistry
Zdroj:	Biochemistry and Cell Biology. 101:125-130
ISSN:	1208-6002 0829-8211
DOI:	10.1139/bcb-2022-0243
Popis:	Multiple studies in Saccharomyces cerevisiae have measured the levels of gene silencing by inserting the URA3 gene at various loci and selecting against URA3-expressing cells by 5-flouroorotic acid (5-FOA). However, 5-FOA affects the cellular pools of dNTPs and can produce side effects. To circumvent this issue, we and others have introduced drug-free techniques to detect silent and active gene states. In this study, we compared three drug-free methods based on the expression of fluorescent reporters in the VIIL telomere of S. cerevisiae. Our results point out that only one of these methods is suitable for large-scale drug-free analyses of gene silencing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6191b28eaced3e3b06603e3d00bfedee https://doi.org/10.1139/bcb-2022-0243 Zobrazit plný text záznamu Plný text ve formátu PDF