Adsorption of two cellobiohydrolases fromTrichoderma reesei to Avicel: Evidence for ?exo-exo? synergism and possible ?loose complex? formation
| Autor: | P. Tomme, M. Claeyssens, V. Heriban |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Chemistry Complex formation Bioengineering General Medicine biology.organism_classification Applied Microbiology and Biotechnology chemistry.chemical_compound Hydrolysis Adsorption Enzyme Exo cellobiohydrolase Sorption isotherm Cellulose Trichoderma reesei Biotechnology Nuclear chemistry |
| Zdroj: | Biotechnology Letters. 12:525-530 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/bf01086347 |
| Popis: | Pure cellobiohydrolases I and II (CBH I & II) fromTrichoderma reesei adsorb strongly (K=104M−1) onto micro-crystalline Avicel. Equilibration is slow (>40 min) and saturation levels determined from the adsorption isotherms are almost identical:107–110 nmoles enzyme/mg Avicel. In admixture synergistic effects are observed dependent on the ratio of the enzymes. These effects are maximal for non-saturating conditions (1–10 μM) and when the enzymes are added in two consecutive steps synergism of binding is only apparent for CBH I. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink