Inhibition of Inducible Nitric Oxide Synthase, Cycleooxygenase-2 and Lipid Peroxidation by Methanol Extract of Pericarpium Zanthoxyli
| Autor: | Sehyung Lee, Jin Uk Oh, Sung-Jin Kim, Jin-Won Chung |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Antioxidant biology medicine.diagnostic_test DPPH medicine.medical_treatment Pharmaceutical Science medicine.disease Nitric oxide Nitric oxide synthase Lipid peroxidation chemistry.chemical_compound Enzyme chemistry Biochemistry Western blot medicine biology.protein Pharmacology (medical) Cell damage |
| Zdroj: | Tropical Journal of Pharmaceutical Research. 12 |
| ISSN: | 1596-9827 1596-5996 |
| DOI: | 10.4314/tjpr.v12i3.15 |
| Popis: | Purpose: To explore the antioxidant properties of the methanol extract of Pericarpium Zanthoxyli and its effect on inducible nitric oxide synthase (iNOS), cycleooxygenase-2 (COX-2) and lipopolysaccharides (LPS)-induced cell damage in macrophage cells. Methods: Anti-oxidant activities were tested by measuring free radical scavenging activity (DPPH, NO) and lipid peroxidation levels. The mechanism of anti-oxidant action of Pericarpium Zanthoxyli extract was determined by Western blot analysis for iNOS and COX-2 expression in LPS-stimulated RAW 264.7 cells. Results: Pericarpium Zanthoxyli extract contained anti-oxidant components including phenolics (2.456 mg/g), flavonoids (0.127 mg/g) and anthocyanins (20.34 mg/g). The extract exerted significant radical scavenging activity in a dose-dependent manner. It also inhibited lipid peroxidation and exerted dramatic reducing power (28.9-fold compared with control at a concentration of 1 mg/ml). Production of iNOS induced by LPS was significantly (p < 0.05) inhibited by the extract, suggesting that the extract inhibits nitric oxide (NO) production by suppressing iNOS expression. Strikingly, COX-2 induced by LPS was also significantly (p < 0.05) inhibited by the extract. Conclusion: These results suggest that the methanol extract of Pericarpium Zanthoxyli exerts significant anti-oxidant activity via inhibiting fr ee radicals, iNOS and lipid peroxidation as well as by inhibition of COX-2 enzyme. |
| Databáze: | OpenAIRE |
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