84 IMMUNE RESPONSE AND BRONCHOPULMONARY BACTERIAL CLEARANCE AFTER MUCOSAL IMMUNIZATION WITH OUTER MEMBRANE PROTEINS (OMP) OF P.AERUGINOSA
| Autor: | Peter Kubesch, J. Freihorst, H. von der Hardt, T. Grundmann, Burkhard Tümmler |
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| Rok vydání: | 1990 |
| Předmět: | |
| Zdroj: | Pediatric Research. 28:290-290 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/00006450-199009000-00108 |
| Popis: | Averting initial colonization of the respiratory tract with P.aeruginosa would be of great benefit for patients with cystic fibrosis (CF). Our approach to this problem is mucosal immunization with a vaccine prepared from the OMP fraction of a PAO-1 strain of P.aeruginosa. Sprague-Dawley rats were given 5 intragastric doses of the vaccine on 5 consecutive days and an intranasal booster dose 21 days later. Immunized animals developed high titers of OMP-specific IgG antibodies in serum and a specific IgA response in bronchoalveolar and small intesinal lavage samples, all determined by ELISA. When challenged 7 days after the booster (day 28) by intratracheal injection of live bacteria of a heterologous strain of P.aeruginosa the immunized rats showed enhanced bronchopulmonary bacterial clearance compared to nonimmunized controls, as indicated by bacterial counts from homogenized lung tissue taken 4 hrs after challenge. Thus, mucosal immunization with OMP vaccines might hinder initial colonisation of the lungs with P.aeruginosa. |
| Databáze: | OpenAIRE |
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