Plant regeneration from mesophyll protoplasts of Agrobacterium rhizogenes-transformed Astragalus melilotoides

Autor:	J. G. Hao, Jing-fen Jia, G. N. Zhang, Xueren Wang, T. He
Rok vydání:	2008
Předmět:	Sucrose Agrobacterium Regeneration (biology) fungi food and beverages Opine Plant Science Horticulture Biology Protoplast biology.organism_classification Transformation (genetics) chemistry.chemical_compound chemistry Shoot Botany medicine Mannitol medicine.drug
Zdroj:	Biologia plantarum. 52:373-376
ISSN:	1573-8264 0006-3134
DOI:	10.1007/s10535-008-0078-4
Popis:	Plant regeneration from mesophyll protoplasts of Agrobacterium rhizogenes-transformed Astragalus melilotoides Pall. was here developed. The protoplasts were isolated directly from the leaves of the hairy root-induced plants. The highest yield of protoplasts was obtained from fully expanded leaves of young plants. Their viability was up to 72 ± 2.3 %. The highest division frequency (32.4 ± 0.13 %) and sustained divisions were obtained in Durand, Potrykus and Donn (DPD) medium supplemented with 2.0 mg dm−3 2,4-dichlorophenoxyacetic acid, 0.2 mg dm−3 6-benzylaminopurine, 0.3 M mannitol, 2 % sucrose and 500 mg dm−3 casein hydrolysate at the plating density of 3.0 × 105 cm−3. The frequency of shoot differentiation from protocalli reached to 91.75 ± 3.1 %. Opine synthesis and polymerase chain reaction analysis confirmed that T-DNA still existed in the protoplast regenerated plants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::615eea7ec5a71bbfd8d8ca668c537029 https://doi.org/10.1007/s10535-008-0078-4 Zobrazit plný text záznamu Full text from SpringerLink