Effect of ozonation on Spirulina platensis filaments by dynamic imaging particle analysis
| Autor: | Roi Peretz, Yael Kinel-Tahan, Yaron Yehoshua, Hadas Mamane, Patricia K. Akao, Vered Cohen-Yaniv |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Algal cells
Lysis Ozone Renewable Energy Sustainability and the Environment Chemistry Algal species 020209 energy Dynamic imaging Forestry 02 engineering and technology Pulp and paper industry Culture growth chemistry.chemical_compound Breakage 0202 electrical engineering electronic engineering information engineering Particle analysis Waste Management and Disposal Agronomy and Crop Science |
| Zdroj: | Biomass and Bioenergy. 127:105247 |
| ISSN: | 0961-9534 |
| DOI: | 10.1016/j.biombioe.2019.05.016 |
| Popis: | Increasing interest in microalgal research calls for simple and automated tools to identify algal species' taxonomic level, examine their quality, monitor their growth, determine their size and shape, and enumerate them. We demonstrate the applicability of micro-flow imaging as an innovative tool to count algal cells and determine their shape and size, toward a future systematic species database, and biofuel applications. Ozonation was used to introduce changes in cell morphology such as cell oxidation, lysis and agglomeration, which were characterized by micro-flow imaging. Two ozone system configurations were used—batch and semi-batch—to oxidize four different Spirulina platensis samples, each presenting a different initial morphology correlated to culture growth stage, environmental conditions and hydration–dehydration of the cells. The ozone dose acted differently in each system: with the batch system (ozone dose of 5–15 mg L−1), the dominant action was breakage of spirulina filaments into smaller ones; with the semi-batch system (ozone dose up to ∼510 mg L−1), the dominant action was perforation of the cell walls and an increase in filament transparency (measured by mean intensity). The study also showed the influence of culture growth stage on ozonated spirulina count and shape analysis. |
| Databáze: | OpenAIRE |
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