Expression of the Male Determining Gene dmrt1bY and Its Autosomal Coorthologue dmrt1a in Medaka
| Autor: | Amaury Herpin, Manfred Schartl, Ute Hornung |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Regulation of gene expression
Genetics 0303 health sciences Embryology Gonad Positional cloning Endocrinology Diabetes and Metabolism Sex reversal Biology Gene expression profiling 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Gene expression medicine Gene Transcription factor 030217 neurology & neurosurgery 030304 developmental biology Developmental Biology |
| Zdroj: | Sexual Development. 1:197-206 |
| ISSN: | 1661-5433 1661-5425 |
| DOI: | 10.1159/000102108 |
| Popis: | Dmrt1bY (aka dmy) has been identified as the master male sex-determining gene of medaka by positional cloning and genetic evidence. How this gene on the molecular level determines the differentiation of the gonad primordium towards a testis, however, is unknown. To better understand the roles of dmrt1bY and its autosomal counterpart dmrt1a in the process of sex determination, a detailed expression analysis by quantitative real-time RT-PCR was performed showing non-organ-specific expression of dmrt1bY during embryogenesis and low expression in adult testes. Surprisingly a high expression of dmrt1bY was noted in the spleen of males. Dmrt1a is exclusively expressed in adult testes, 50 times higher than dmrt1bY. Dmrt1bY mRNA and protein have short half-lives and the GFP fusion protein products of both dmrt1 genes are localized to the nucleus. These features are consistent with the suggestion that Dmrt1 proteins might function as DNA-binding transcription factors. |
| Databáze: | OpenAIRE |
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