Simple Fluorescence Turn-On Chemosensor for Selective Detection of Ba2+ Ion and Its Live Cell Imaging
| Autor: | Kwan Seob Shim, Sivakumar Allur Subramaniyan, Palanisamy Ravichandiran, Dong Jin Yoo, Antony Paulraj Bella, Ae Rhan Kim, Princy Merlin Johnson |
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| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Sulfonyl chemistry.chemical_classification HEPES 010401 analytical chemistry 010402 general chemistry Photochemistry 01 natural sciences Fluorescence Binding constant 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Live cell imaging Benzamide Phenoxazine |
| Zdroj: | Analytical Chemistry. 91:10095-10101 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.9b02057 |
| Popis: | A phenoxazine-based fluorescence chemosensor 4PB [(4-(tert-butyl)-N-(4-((4-((5-oxo-5H-benzo[a]phenoxazin-6-yl)amino)phenyl)sulfonyl)phenyl)benzamide)] was designed and synthesized by a simple synthetic methods. The 4PB fluorescence chemosensor selectively detects Ba2+ in the existence of other alkaline metal ions. In addition, 4PB showed high selectivity and sensitivity for Ba2+ detection. The detection limit of 4PB was 0.282 μM and the binding constant was 1.0 × 106 M-1 in CH3CN/H2O (97.5:2.5 v/v, HEPES = 1.25 mM, pH 7.3) medium. This chemosensor functioned through the intramolecular charge transfer (ICT) mechanism, which was further confirmed by DFT studies. Live cell imaging in MCF-7 cells confirmed the cell permeability of 4PB and its capability for specific detection of Ba2+ in living cells. |
| Databáze: | OpenAIRE |
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