Porcine milk induces a strengthening of barrier function in porcine jejunal epithelium in vitro
Autor: | Alexander G. Markov, Robert Pieper, Silke S. Zakrzewski, Judith Radloff, Salah Amasheh |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Tight junction Ussing chamber General Neuroscience Biology General Biochemistry Genetics and Molecular Biology In vitro Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine History and Philosophy of Science Biochemistry Permeability (electromagnetism) 030220 oncology & carcinogenesis Paracellular transport Claudin Incubation Barrier function |
Zdroj: | Annals of the New York Academy of Sciences. 1397:110-118 |
ISSN: | 0077-8923 |
DOI: | 10.1111/nyas.13340 |
Popis: | Milk contains a variety of components that have been shown to affect the expression and localization of epithelial tight junction proteins and therefore the intestinal barrier. Thus, we hypothesized that milk would have an effect on intestinal barrier properties, owing to effects on the tight junction in an intraspecies porcine intestinal in vitro model. Jejunal samples of piglets derived from different age groups were analyzed. Transepithelial electrical resistance was recorded employing the Ussing chamber technique. Porcine milk or predigested milk in buffer solution was added to the apical side, and effects were compared to untreated controls. Unidirectional paracellular flux measurements were performed using sodium fluorescein. Tight junction protein expression and localization were analyzed by immunoblotting and immunofluorescence microscopy. Incubation with milk or predigested milk led to an increase in transepithelial electrical resistance, while paracellular permeability for sodium fluorescein did not result in significant changes. Densitometric analysis of immunoblot signals did not show significant alterations in claudin expression, but a reduction of claudin signals in apicolateral membrane compartments in both approaches became apparent via immunohistology. The functional effect might reflect a physiological protective mechanism, when offspring exclusively rely on their mother's milk and are exposed to a plethora of potentially barrier-perturbing factors. |
Databáze: | OpenAIRE |
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