| Popis: |
We produced the transgenic silkworm that expressed the enhanced blue fluorescent protein (EBFP) in the cocoon ofsilkworms. The EBFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed tobe secreted into the lumen of the posterior silk glands. The expression of the EBFP/H-chain fusion gene was regulatedby the fibroin H-chain promoter. The use of the 3×P3-driven DsRed2 cDNA as a marker allowed us to rapidly dis-tinguish transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 300 eggs of silkworms,Baegokjam. We obtained 5 broods. The cocoon displayed blue fluorescence, proving that the fusion protein was presentin the cocoon. Also, the presence of fusion proteins in cocoons was demonstrated by SDS-PAGE and western blot anal-ysis. Accordingly, we suggest that the EBFP fluorescence silk will enable the production of the silk-based biomaterials. Key words : Fluorescent, Transgenic silkworm, EBFP, Bombyx mori, Silk |
