Expression of decorin and biglycan by rabbit articular chondrocytes

Autor: Magali Demoor-Fossard, Françoise Rédini, Jean-Pierre Pujol, Martine Boittin
Rok vydání: 1998
Předmět:
Zdroj: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1398:179-191
ISSN: 0167-4781
DOI: 10.1016/s0167-4781(98)00044-x
Popis: In this study, the levels of mRNAs coding for aggrecan, decorin and biglycan in rabbit articular chondrocytes were investigated, using both monolayer and 3D-alginate cultures treated with TGF-β1 and IL-1β. The cells were shown to express higher amounts of proteoglycan messages, specially the aggrecan, in gels than in monolayers. TGF-β1 increased aggrecan mRNA in both systems, whereas biglycan message was elevated only in alginate. It markedly decreased decorin expression in monolayer, either in primary or passaged cultures. In contrast, IL-1β had a weak inhibitory effect on both decorin and biglycan expression. Subculturing induced a dramatic decrease of aggrecan mRNA, while that of decorin augmented. Biglycan expression transiently increased after two passages, whereas it declined in further subcultures. Passaged chondrocytes transferred to alginate re-expressed high levels of aggrecan, decorin and biglycan. The data point to the influence of morphology, proliferative state and environment of the articular chondrocytes on their biosynthetic responses to cytokines. Although these immature cells do not fully reflect the adult chondrocytes present in the cartilage, this study may help in understanding the behaviour of these cells in osteoarticular diseases, where the surrounding extracellular matrix is profoundly altered.
Databáze: OpenAIRE
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