Metal ion-catalysed hydrolysis of ampicillin in microbiological growth media
| Autor: | Martin N. Hughes, Robert K. Poole, Steven J. Beard, Diana T. Ciccognani |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Metal ions in aqueous solution
Inorganic chemistry chemistry.chemical_element Zinc biochemical phenomena metabolism and nutrition Bacterial growth Microbiology Copper Metal Hydrolysis Anodic stripping voltammetry chemistry Ampicillin visual_art Genetics medicine visual_art.visual_art_medium Molecular Biology medicine.drug Nuclear chemistry |
| Zdroj: | FEMS Microbiology Letters. 96:207-211 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1992.tb05418.x |
| Popis: | Anodic stripping voltammetry of bacterial growth medium containing copper(II) and ampicillin shows that Cu(II) is complexed by the antibiotic and that this complex decomposes to give Cu(II) complexes with ligands derived from ampicillin. At pH 7, substantial decomposition of ampicillin occurs over a few minutes, and even the very low levels of Cu(II) in Chelex-extracted medium are able effectively to catalyse the decomposition. The significance of this observation was shown during the screening of an Escherichia coli cosmid library for clones exhibiting increased resistance to Zn(II), Co(II) or Cd(II): the unexpected growth of the ampicillin-sensitive host E. coli strain on Luria-Bertani plates containing ampicillin and any of these metals was attributed to metal ion-catalysed decomposition of ampicillin. The instability of ampicillin (and other β-lactam antibiotics) to metal ion-catalysed hydrolysis means that great care must be taken to ensure that such reactions do not occur in growth media. Furthermore, it is clear that double selection for resistance to ampicillin and metals such as Cu(II), Zn(II), Co(II) and Cd(II) is impossible. |
| Databáze: | OpenAIRE |
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