Enzyme-assisted Extraction and Enrichment of Galanthamine from Lycoris aurea
| Autor: | Liu Xiaopan, Tian Chunlian, Ke Song, Wang Peng, Zhuo-bing Xiao, Ji-xin Qin |
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| Rok vydání: | 2016 |
| Předmět: |
Pharmacology
Chromatography Traditional medicine biology Chemistry Cellulase 01 natural sciences 0104 chemical sciences Solvent 010404 medicinal & biomolecular chemistry 03 medical and health sciences 0302 clinical medicine Adsorption Complementary and alternative medicine Enzymatic hydrolysis Reagent Desorption biology.protein Pharmacology (medical) Pectinase Ion-exchange resin 030217 neurology & neurosurgery |
| Zdroj: | Chinese Herbal Medicines. 8:182-188 |
| ISSN: | 1674-6384 |
| DOI: | 10.1016/s1674-6384(16)60028-x |
| Popis: | Objective To explore the optimum condition for complex enzyme-assisted extraction of galanthamine from Lycoris aurea by L 9 (3 4 ) orthogonal array design and separation effect of cation exchange resin on galanthamine. Methods Cellulase and pectinase solution was used as the extraction solvent. The effects of pH value of enzyme, amount of complex enzyme, dissociation time, and enzymatic hydrolysis temperature on the extraction results were investigated. Results The optimal conditions were obtained as follows: ratio of solid to liquid (g: mL) 1:10, pH value 4.5, amount of complex enzymes 4%, enzymatic hydrolysis temperature 50 °C, and reaction time 2.0 h. Under these conditions, the extraction yield of galanthamine was 0.0294%. In addition, D-001 cation exchange resin was selected for separation of galanthamine. The separation conditions were that adsorption flow rate was 3 BV/h with reagent of pH 2 and the desorption flow rate was 3 BV/h with 70% ethanol solution containing 1.5 mol/L ammonia. After separation, the content of galanthamine was increased to 12.31%. Conclusion The results provide a reference for industrial production of galanthamine. |
| Databáze: | OpenAIRE |
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