SEPARATION AND ANALYSIS OF AMLODIPINE/BENAZEPRIL COMBINATION IN CAPSULES BY A NOVEL ION PAIR LIQUID CHROMATOGRAPHY
| Autor: | Saleh Trefi, Yaser Bitar |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Pharmacology
Materials science Chromatography Amlodipine/benazepril 010401 analytical chemistry Cetrimide Pharmaceutical Science Linearity Benazepril 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences High-performance liquid chromatography 0104 chemical sciences chemistry.chemical_compound chemistry Phase (matter) medicine Amlodipine 0210 nano-technology Acetonitrile medicine.drug |
| Zdroj: | International Journal of Pharmacy and Pharmaceutical Sciences. 11:107 |
| ISSN: | 0975-1491 |
| DOI: | 10.22159/ijpps.2019v11i1.29672 |
| Popis: | Objective: The objective of this study was to develop and validate a novel ion-pair liquid chromatography method, in order to separate and assay of amlodipine/benazepril combination in capsules. This method was a fast, practical and additional choice in quality control laboratories. Methods: The chromatographic conditions comprised of a classical C 18 -type stationary phase (250 × 4.6 mm, 5μ), with a mobile phase consisting of: 45% of 10 - 3 M of cetrimide and 55% acetonitrile. The flow rate was 1 ml/min; the detection wavelength was at 242 nm, under ambient temperature. Results: The method was validated for linearity with correlation coefficients very close to one, the accuracy with mean recovery values between 95.0-105.0%, precision with relative standard deviations of the calculated concentrations less than 5.0% and specificity in the presence of degradation products and excipients. Conclusion: The results presented in this paper showed that the developed method was fast and applicable, for the separation and determination of amlodipine/benazepril combination in capsules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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