Inhibitory activity of Crude and Purified Extracts of Datura inoxia against Some Clinically Isolated Bacteria
| Autor: | Manodeep Sen, Priti Mathur, Chandni Tandon |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
biology
Pseudomonas aeruginosa Chemistry medicine.drug_class Antibiotics medicine.disease_cause biology.organism_classification Datura inoxia Microbiology Staphylococcus aureus medicine Pharmacology (medical) Antibacterial activity Pharmacology Toxicology and Pharmaceutics (miscellaneous) Staphylococcus Escherichia coli Bacteria |
| Zdroj: | Research Journal of Pharmacy and Technology. 11:695 |
| ISSN: | 0974-360X 0974-3618 |
| DOI: | 10.5958/0974-360x.2018.00131.2 |
| Popis: | The alarming incidence of bacterial resistance even in regions with improved healthcare systems has accelerated the need to search for alternative antibacterial therapies. Considering this, hexane, ethanol, aqueous, methanol and chloroform leaf extract of Datura inoxia were screened for their antibacterial activity against the clinical strains of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli isolated from blood, urine and pus. Agar well diffusion assay was employed to detect inhibitory activity of the plant. Ethanolic extract was found most effective among all the tested extracts. The extract showed greater inhibitory activity against the strains of Staphylococcus aureus. Ethanolicextract was further subjected for fractionation through column chromatography. Different isolated fractions were again screened for their antibacterial activity against the strains of Staphylococcus aureus. All the fractions showed inhibitory activity, of which fraction 4 was found effective against all the tested strains. Present study also revealed that, both gram negative and gram positive bacterial isolates from urine samples were more inhibited by the ethanolic extract than the isolates from pus and blood samples. Further studies are needed to explore theantibacterial activity of this ethanolic extract in various urinary tract infections, which presently require long term treatment with synthetic antibiotics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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