Development of HPLC-Method for Simultaneous Determination of API and Related Components in Thiowurtzine: A New Non-Narcotic Analgesic

Autor:	S. V. Sysolyatin, Dmitry Novikov, Dmitry Kurgachev, D. A. Kulagina, Eugenia Tomilova, Krylova Svetlana G
Rok vydání:	2021
Předmět:	Isocratic elution Chromatography 010405 organic chemistry Chemistry 010401 analytical chemistry Organic Chemistry Clinical Biochemistry Analgesic 01 natural sciences Biochemistry Rapid detection 0104 chemical sciences Analytical Chemistry Non narcotic Hplc method
Zdroj:	Chromatographia. 84:147-154
ISSN:	1612-1112 0009-5893
DOI:	10.1007/s10337-020-03993-x
Popis:	Thiowurtzine, a hexaazaisowurtzitane derivate, is a novel original non-opioid analgesic. In this article, an HPLC–UV method has been developed for rapid detection and quantification of API and related substances contained in thiowurtzine capsules. Six thiowurtzine impurities were separated successfully from each other by isocratic elution using a Luna 5u PFP (2) (150 mm × 4.6 mm, 5 µm) column with 30 µL injection volumes at a wavelength of 254 nm. Commercially unavailable impurities were formed by degradation of thiowurtzine and preliminarily identified by high-resolution LC–MS. This method proved to be robust, linear, repeatable, sensitive, selective and handy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::60f6c1065a67f3e56de20563c15ad89c https://doi.org/10.1007/s10337-020-03993-x Zobrazit plný text záznamu Full text from SpringerLink