Isolation and culture of mesophyll protoplast from apricot
| Autor: | Lorenzo Burgos, F. Ortín-Párraga |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Chromatography fungi food and beverages biochemical phenomena metabolism and nutrition Horticulture Biology Protoplast Plasmolysis Incubation period chemistry.chemical_compound Enzyme chemistry Botany Genetics bacteria Agarose Composition (visual arts) Centrifugation Digestion |
| Zdroj: | The Journal of Horticultural Science and Biotechnology. 78:624-628 |
| ISSN: | 2380-4084 1462-0316 |
| DOI: | 10.1080/14620316.2003.11511674 |
| Popis: | SummaryYields of 106–107 apricot mesophyll protoplasts g fw–1 were obtained depending on factors such as plasmolysing pretreatment, digesting enzymes and digestion time. Onozuka R-10 (1%) in combination with Pectolyase Y-23 (0.1%) and Hemicellulase (1%) was found best for protoplast isolation among several enzyme combinations tested. Viability was 83% with this enzyme combination. Plasmolysis of leaves for 90 min in a 13% sorbitol solution greatly increased the number of protoplasts obtained. Optimum incubation time of 13–16 h produced the best combination of yield and viability. During the purification of protoplasts a critical step was the centrifugation of the sucrose gradient at 75 g with a dramatic decrease in the recovery of protoplasts at lower and higher centrifugation speeds. Protoplasts were cultured under different media composition and growth regulator combinations. Limited growth and division of protoplasts embedded in agarose drops were obtained. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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