Activation of Bovine NK cells through Toll-like receptors (81.1)
| Autor: | Sandra V Mayer, William Golde, Don Mark Estes |
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| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 182:81.1-81.1 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Toll like receptors (TLRs) play a critical role in the immediate detection and control of infection in mammals and are very important for initiating a series of signaling events which result in proinflammatory gene expression. Many of these receptors are type I transmembrane proteins that belong to the interleukin-1 receptor family. There are 13 TLRs already identified in mammals, 10 in humans, 10 in bovine and 13 in mice. The TLRs main functions include secretion of type 1 interferon, proinflamatory cytokines, chemokines and expression of co-stimulatory molecules. The aim of this study was to verify intracellular perforin and gamma interferon production, granzyme, perforin transcription and extra cellular gamma interferon secretion in bovine NK cells treated with TLRs ligands such as TLR2, TLR3, TLR4, TLR5 and TLR9. The cells were sorted by magnetic cell sorting with the NKp46 antibody and 24 hours later they were stimulated for 72 hours with the TLR ligands mentioned above. Our preliminary results demonstrated that flagellin (6/6) and PPD (5/6) are the most inducers of intracellular interferon gamma production followed by LPS (4/6) and CpG (4/6). When we analyzed the intracellular perforin production we verified an increase in the cells treated with CpG (4/6) or LPS (3/6). These results suggest that bovine NK cells might be activated by these TLRs ligants, otherwise further studies are needed to elucidate this hypothesis. Keywords: Toll like receptors, bovine, NK cells. |
| Databáze: | OpenAIRE |
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