Chemical Engineering Thermodynamics and Protein Adsorption Chromatography

Autor:	Jørgen Mollerup
Rok vydání:	2013
Předmět:	Chromatography General Chemical Engineering Thermodynamics General Chemistry Oligomer Separation process Thermodynamic model Chromatographic separation chemistry.chemical_compound Adsorption chemistry Scientific method Process simulation Protein adsorption
Zdroj:	Journal of Chemical & Engineering Data. 59:991-998
ISSN:	1520-5134 0021-9568
DOI:	10.1021/je400739j
Popis:	The process of chromatographic separation and purification of recombinant products is of singular importance to the biopharmaceutical industries because it is the only separation process that can deliver high-purity products. A prerequisite for the application of process simulation in the development of chromatographic separations is the quality of the thermodynamic models of adsorption equilibria. The first part of the paper outlines the general thermodynamic principles of protein adsorption equilibria using well-established thermodynamic concepts, theories, and models. In the second part of the paper we present four examples. The first example shows how oligomer formation will modify a chromatographic run. In the second example we explore the separation potential of oligomer formation in the design of a chromatographic purification step. The third example demonstrates how synergies in the thermodynamic model development can be obtained by combining disparate experiments, here, chromatographic experiment...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::608ed5d4cb9bb5b99fb9d85a8647a10d https://doi.org/10.1021/je400739j Zobrazit plný text záznamu