| Autor: |
Werner C, Andreas T. Sørensen, Ulrik Gether, Guthrie Da, Freja Herborg, Ejdrup Al, Jonatan Fullerton Støier, Sauer M, Haahr No, Amy Hauck Newman, Lycas, Søren H. Jørgensen |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.03.09.434538 |
| Popis: |
The nanoscopic organization and regulation of individual molecular components in presynaptic varicosities of neurons releasing modulatory volume neurotransmitters like dopamine (DA) remain largely elusive. Here we show by application of several single-molecule sensitive super-resolution microscopy techniques to cultured neurons and mouse striatal slices, that the dopamine transporter (DAT), a key protein in varicosities of dopaminergic neurons, exists in the membrane in dynamic equilibrium between an inward-facing nanodomain-localized and outward-facing unclustered configuration. The balance between these configurations is inversely regulated by excitatory drive and by DA D2-autoreceptor activation in manner dependent on Ca2+-influx via N-type voltage-gated Ca2+-channels. The DAT nanodomains contain tens of transporters molecules and overlap with nanodomains of PIP2 (phosphatidylinositol-4,5-bisphosphate) but show little overlap with D2-autoreceptor, syntaxin-1 and clathrin nanodomains. By demonstrating that nanoscopic reorganizations with putative major impact on transmitter homeostasis can take place in dopaminergic varicosities, the data have important implications for understanding modulatory neurotransmitter physiology. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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