Abstracts: Natural moisturizing factors in the stratum corneum I. Effects of lipid extraction and soaking
| Autor: | Angela A. LaRuffa, Marisa Robinson, Marty O. Visscher, R Randall Wickett |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
Aging Chromatography integumentary system Extraction (chemistry) Pharmaceutical Science Dermatology High-performance liquid chromatography Amino acid Desquamation chemistry.chemical_compound Colloid and Surface Chemistry medicine.anatomical_structure chemistry Chemistry (miscellaneous) Drug Discovery medicine Stratum corneum Perchloric acid medicine.symptom Water binding Filaggrin |
| Zdroj: | International Journal of Cosmetic Science. 32:394-394 |
| ISSN: | 0142-5463 |
| Popis: | pp. 13–22 Natural moisturizing factor (NMF) is essential for appropriate stratum corneum hydration, barrier homeostasis, desquamation, and plasticity. It is formed from filaggrin proteolysis to small, hygroscopic molecules including amino acids. We hypothesized that common lipid extraction and soaking in water would alter the level of NMF in the upper stratum corneum (SC) and its biophysical properties. A novel method of measuring and quantifying the amino acid components of NMF is presented. Adhesive tapes were used to collect samples of the SC and were extracted with 6 mM perchloric acid for analysis by reverse-phase high-performance liquid chromatography (HPLC).The HPLC results were standardized to the amount of protein removed by the tapes. An increase in NMF was found with increased SC depth. Also, the combination of extraction and soaking was found to increase NMF loss relative to control or to extraction or soaking alone. Our results indicate that common skin care practices significantly influence the water binding materials in the upper SC. The findings have implications for the evaluation and formulation of skin care products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|