14 Sperm membrane integrity in ejaculates from young bulls may be improved by single-layer centrifugation
| Autor: | Jane M. Morrell, Anders Johannisson, E. Hurri, I. Lim-Verde |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
endocrine system
urogenital system Extender Semen Reproductive technology Biology Sperm Cryopreservation law.invention Andrology Endocrinology Reproductive Medicine law Reproductive biology Genetics Animal Science and Zoology Molecular Biology Spermatogenesis reproductive and urinary physiology Fertilisation Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 31:133 |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/rdv31n1ab14 |
| Popis: | Sperm quality in the first ejaculates of young bulls is often poor, leading to rejection of these samples for commercial freezing. It may be several weeks before sperm quality reaches the thresholds regarded as being acceptable for commercial production. Single-layer centrifugation (SLC) has been shown to separate robust bull spermatozoa from the rest of the ejaculate (Goodla et al. 2015 J. Dairy Sci. 97, 2204-2212; Nongbua et al. 2017 Reprod Domest. Anim. 52, 596-602). The aim of this study was to test whether SLC can be used to select spermatozoa from the ejaculates of young bulls and thus freeze samples earlier than is currently possible. Ejaculates (at least 3 per bull; n=33) were collected from 9 young Holstein or Swedish Red bulls, 255 to 415 days old. The sperm concentration was adjusted to 69×106 spermatozoa mL−1 with Andromed™ (Minitub, Tiefenbach, Germany) before layering part of the sample over 4mL of Bovicoll colloid. The remaining sample served as the unselected control. After centrifugation at 300×g for 20min, the sperm pellet was aspirated into fresh extender. Both control (uncentrifuged) and SLC samples were frozen and stored in LN pending analysis. Membrane integrity was evaluated by flow cytometry after staining for 10min at 37°C with SYBR14/propidium iodide (Live-Dead Sperm Viability Kit, L-7011; Invitrogen, Carlsbad, CA, USA). Paired t-test was used to compare results for control and SLC samples, and Pearson correlation was used for age and membrane integrity. The median age at which the samples were taken was 319 days (range: 255-415 days). Membrane integrity varied considerably among bulls (range: 11-72%). The mean proportion of membrane-intact spermatozoa in controls and SLC samples was 40±15 and 47±16%, respectively (P |
| Databáze: | OpenAIRE |
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