| Autor: |
Eva Aldová, Antonín Paleček, Richard Kahlich, Jiří šourek |
| Rok vydání: |
1985 |
| Předmět: |
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| Zdroj: |
Systematic and Applied Microbiology. 6:82-85 |
| ISSN: |
0723-2020 |
| DOI: |
10.1016/s0723-2020(85)80016-3 |
| Popis: |
Summary A simple modification of Campylobacter jejuni serotyping based exclusively on using fresh native cultures grown on Brucella blood agar at 42 °C for 40 h was devised. Six increasing intravenous doses of live culture suspensions injected into rabbits at weekly intervals were tolerated without any undesirable reactions and resulted in the production of high — titre antisera. Suspensions of cultures were invariably homogeneous, did not autoagglutinate and reacted on slides with antisera by clear-cut granular agglutination. In contrast to human convalescent sera, with cross- agglutinated strains from different patients, rabbit antisera gave monospecific reactions not only after absorption but even after mere 1 : 8 dilution. Even in the native state none of them agglutinated control C. fetus strains. The antigenic essence of these reactions was not studied. A provisional set of 13 typing sera was prepared. A group of 60 C. jejuni isolates from human and animal sources displayed a predominance of 6 types. Two campylobacteriosis outbreaks were investigated and found to have been caused by the same type. The technical simplicity, high discriminative power and reproducibility, as well as the utilization of the native structure of the agent make the proposed method an attractive tool for practical study of the epidemiological features of campylobacteriosis in Czechoslovakia. So far direct comparibility of types with the typing system of other authors is not claimed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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