The use of polymerase chain reaction to detect latent infection of Clavibacter michiganensis subsp. michiganensis in tomato seedlings

Autor:	N. Fiore, R. Ghedini
Rok vydání:	1995
Předmět:	medicine.medical_specialty biology Plant Science Horticulture biology.organism_classification Microbiology law.invention Seedling law Molecular genetics medicine Routine analysis Agronomy and Crop Science Clavibacter michiganensis Polymerase chain reaction
Zdroj:	EPPO Bulletin. 25:449-454
ISSN:	1365-2338 0250-8052
DOI:	10.1111/j.1365-2338.1995.tb00578.x
Popis:	A method was developed for routine analysis to detect latent infection of Clavibacter michiganensis subsp. michiganensis in samples from tomato seedling lots, before transplant, using polymerase chain reaction. In samples of 300 stem segments 1 cm long, the sensitivity threshold of the method was estimated at around 1.1 × 103 corynebacteria or 0.33% of latently infected stems. This method was shown to have a good specificity.
Databáze:	OpenAIRE
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