UPLC-MS Analysis of Cannabis sativa Using Tetrahydrocannabinol (THC), Cannabidiol (CBD), and Tetrahydrocannabinolic Acid (THCA) as Marker Compounds: Inhibition of Breast Cancer Cell Survival and Progression
| Autor: | Motlalepula G. Matsabisa, Sunelle Rademan, Khorommbi Ndivhuwo Kevin, Asis Bala, Vinesh Maharaj |
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| Rok vydání: | 2019 |
| Předmět: |
Pharmacology
0303 health sciences Chemistry Plant Science General Medicine medicine.disease Cannabis sativa 03 medical and health sciences 0302 clinical medicine Breast cancer Complementary and alternative medicine 030220 oncology & carcinogenesis Drug Discovery Tetrahydrocannabinolic acid medicine Uplc ms ms Breast cancer cells Tetrahydrocannabinol Cannabidiol 030304 developmental biology medicine.drug |
| Zdroj: | Natural Product Communications. 14:1934578X1987290 |
| ISSN: | 1555-9475 1934-578X |
| Popis: | Cannabis sativa L. extracts were characterized by ultra performance liquid chromatography-mass spectrometry (UPLC-MS) using tetrahydrocannabinol (THC), cannabidiol (CBD), and tetrahydrocannabinolic acid (THCA) as marker compounds. The inhibitory effects of various extracts were determined on the survival and progression of highly metastatic breast cancer cells. A higher amount of CBD was found in the dichloromethane extract, and this was found to be effective in inhibiting breast cancer cell growth in vitro and in angiogenesis. Collectively, it may be concluded that CBD, THC, and THCA in the African variety of C. sativa can be used as marker compounds in UPLC-MS analysis. The ability of the plant to inhibit breast cancer cell survival and progression may affirm the traditional use of the drug as an anticancer agent. |
| Databáze: | OpenAIRE |
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