Preparation of bioactive amide compounds from black pepper by countercurrent chromatography and preparative HPLC

Autor:	Yongyang Jin, Qizhen Du, Dengyong Qian
Rok vydání:	2013
Předmět:	chemistry.chemical_compound Preparative hplc Countercurrent chromatography Chromatography Chemistry Piperine Yield (chemistry) Amide Pepper Organic chemistry Nuclear magnetic resonance spectroscopy Agronomy and Crop Science High-performance liquid chromatography
Zdroj:	Industrial Crops and Products. 44:258-262
ISSN:	0926-6690
Popis:	Amide compounds are the major bioactive substances in black pepper. Black pepper extracts were fractionated by high-speed countercurrent chromatography resulting in three compounds: dehydropipernonaline, piperine and (2E,4E,12Z)-N-isobutyl-octadecatrienamide, and four fractions which were separated by preparative HPLC to yield ten purified compounds: retrofractamide-A, (2E,4E)-N-isobutyl-decadienamide, retrofractamide-C, piperoleine A, dehydropiperoleine A, retrofractamide-B, piperoleine B, pipernonaline, pipercyclobutanamides A and (2E,4E,14Z)-N-isobutyleicosa-2,4,14-trienamide as identified by ESI-MS and NMR analysis. The results of the present study indicated that HSCCC is an effective technique for the preparation of bioactive amide compounds from black pepper.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::602d70c2a54371ab91bb29b00db5c389 https://doi.org/10.1016/j.indcrop.2012.11.019 Zobrazit plný text záznamu Full Text from ScienceDirect