| Autor: |
Rhys Dowler, Uwe Ortmann, Marcelle König, Rainer Erdmann, Felix Koberling, Sumeet Rohilla, Benedikt Krämer, Mariano Gonzalez Pisfil |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Single Molecule Spectroscopy and Superresolution Imaging XIII. |
| DOI: |
10.1117/12.2542417 |
| Popis: |
Scanning FCS (sFCS) is a great tool for studying slowly diffusing species as is often the case in cell membranes. In sFCS, the excitation volume is scanned rapidly through the sample allowing for simultaneous measurement at multiple locations. The shorter residence times also lead to lower photon doses experienced by each detected molecule, reducing the risk of photobleaching. Here, we show results from sFCS measurements on supported lipid bilayers (SLBs) where fluorescence lifetime information is used to achieve an axial nanometric localization based on Metal Induced Energy Transfer (MIET). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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