| Autor: |
Loganathan Doraisamy, Seung Lee, Cathy A. Petti, Charlene Wong, Suresh M. Menon, Rosemary C. She, Katherine Park, Paul Yang, Sara Hash, Jonas Petterson, Pamela Ward |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
The Journal of Molecular Diagnostics. 19:230-235 |
| ISSN: |
1525-1578 |
| DOI: |
10.1016/j.jmoldx.2016.09.012 |
| Popis: |
We evaluated the performance of an early prototype core molecular mirroring nuclear magnetic resonance detection platform (Mentor-100) to detect toxigenic Clostridium difficile from stool. This technology uses customized nanoparticles bound to target specific oligonucleotide probes that form binaries in the presence of nucleic acid from the target microorganism. Liquid patient stool specimens were seeded with C. difficile or other Clostridium species to determine the analytical sensitivity and specificity. Samples underwent nucleic acid extraction and target amplification with probes conjugated with iron nanoparticles. Signal from nuclear magnetic resonance spin–spin relaxation time was measured to detect the presence or absence of toxigenic C. difficile. The limit of detection was |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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