The Role of Apoptosis in the Resolution of T Cell-Mediated Cutaneous Inflammation
| Autor: | Catherine H. Orteu, Len W. Poulter, Malcolm H. A. Rustin, Caroline A. Sabin, Mike Salmon, Arne N. Akbar |
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| Rok vydání: | 1998 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 161:1619-1629 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.161.4.1619 |
| Popis: | We have investigated cutaneous purified protein derivative-induced delayed-type hypersensitivity (DTH) responses in healthy volunteers to determine features associated with both the generation and resolution of the reaction. The clinical peak of the response occurred at day 3; however, T cell numbers were maximal on day 7. There was a preferential increase of CD4+CD45RO+ T cells on day 7, which was largely due to proliferation, since a mean of 19% was in cycle. The proliferation of this subset was associated with the presence of IL-15, which was expressed as early as 12 h, and IL-2, which showed peak expression at 7 days. By day 14, there was a significant decrease in both the mean T cell number/unit area and IL-2 and IL-15 expression in perivascular infiltrates. Maximal CD95 (Fas/Apo-1) ligand and TNF-α expression were observed at 7 days and were associated with the presence of 1.83% (range 0.81–2.48%) apoptotic T cells. At 14 days, CD95 ligand and TNF-α expression were reduced significantly, and the presence of 2.5% (range 1.5–3.75%) of apoptotic T cells at this time was probably due to cytokine deprivation, associated with decreased Bcl-2 relative to Bax expression. The induction and resolution of the Mantoux reaction may depend on the expression of cytokines, such as IL-2 and IL-15, which regulate both proliferation and apoptosis in T cells. Failure to control either of these phases of the Mantoux reaction may contribute to the chronicity of inflammatory responses in certain cutaneous diseases. |
| Databáze: | OpenAIRE |
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