Stabilization of bilayer lipid membranes on solid supports by trehalose
| Autor: | H.T. Tien, Tibor Hianik, B. Sivak, M. Gyeppessova, A. Ottova-Leitmannova, J. Dlugopolsky |
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| Rok vydání: | 1996 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Materials science Electrostriction urogenital system Biophysics nutritional and metabolic diseases Lipid bilayer mechanics Electrolyte Trehalose chemistry.chemical_compound Crystallography Membrane chemistry Chemical engineering Phosphatidylcholine Electrochemistry Lipid bilayer phase behavior Physical and Theoretical Chemistry Elastic modulus |
| Zdroj: | Bioelectrochemistry and Bioenergetics. 39:299-302 |
| ISSN: | 0302-4598 |
| DOI: | 10.1016/0302-4598(95)01862-x |
| Popis: | Using the electrostriction method the effect of the glucose and trehalose on the elasticity modulus perpendicular to the membrane plane, E ⊥ , and the electrical capacitance, C , of supported bilayer lipid membranes (s-BLM) formed on the freshly cut tip of Teflon-coated Ag wire was studied. Addition of saccharides into the electrolyte resulted in a decrease in the elasticity modulus of the s-BLM formed from the soybean phosphatidylcholine in n-hexadecane, while the capacitance was increased. In addition, the trehalose has a considerable stabilizing effect on the above parameters of the s-BLM. Treatment of the s-BLM in an electrolyte containing 0.3 M of the trehalose allowed storage of the s-BLM under dry conditions and under refrigeration, with the subsequent recovery of membrane parameters after the wire had been dipped into the electrolyte. |
| Databáze: | OpenAIRE |
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