Analogs of human embryonic prealbumin-2 in animals

Autor:	D. M. Falaleeva, V. V. Kalashnikov, Yu. S. Tatarinov
Rok vydání:	1981
Předmět:	Fetus Amniotic fluid biology medicine.diagnostic_test Embryogenesis General Medicine Immunofluorescence Molecular biology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Blood serum chemistry biology.protein medicine Glutaraldehyde Antibody Cellular localization
Zdroj:	Bulletin of Experimental Biology and Medicine. 92:928-930
ISSN:	1573-8221 0007-4888
DOI:	10.1007/bf00837001
Popis:	The cellular localization of EPA-2 in tissues of calf fetuses and adult animals was studied by the indirect immunofluorescence analysis method. Tissue sections 4-8 ~ thick were fixed with alcohol and acetic acid [7] and embedded in paraffin wax [8]. The sections were labeled by means of antibodies against EPA-2 obtained with the aid of an immunosorbent. EPA-2 immobilized on ACA-34 ultrogel by means of glutaraldehyde [6] was used as the immunosorbent. The technique of immunofluorescence analysis was described by the writers previously [2]. To study analogs, amniotic fluid, tissue, and blood serum from fetuses in the first half of embryonic development, and tissue and blood serum from adult animals were chosen. The tissue extracts were prepared in Tris-glycine buffer, pH 8.3, with the addition of detergents [4].
Databáze:	OpenAIRE
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