Abstract 623: Role Of Cd73-derived Adenosine In Atherosclerosis
| Autor: | Anja Buchheiser, Barbara Emde, Jens W Fischer, Jürgen Schrader |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Circulation. 116 |
| ISSN: | 1524-4539 0009-7322 |
| DOI: | 10.1161/circ.116.suppl_16.ii_114 |
| Popis: | We recently reported that mice with targeted deletion of ecto-5′-nucleotidase (CD73) are characterized by enhanced platelet activation and increased adherence of monocytes to the endothelium. Wire-induced injury of the carotid artery resulted in enhanced neointima formation associated with increased macrophage content and VCAM-1 expression in CD73−/−mice. In order to study a possible modulation of atherogenesis and vascular inflammation by CD73-derived adenosine, we generated ApoE−/−/CD73−/− double mutant mice. Quantification and characterisation of atherosclerotic lesions was performed in WT, ApoE−/−, and ApoE−/− / CD73−/− mice on chow diet after 6 and 12 month, respectively, by macroscopic analysis of the descending thoracic aorta after oil red staining and by histological stainings for cholesterol, hyaloronic acid, and collagen in cryo sections of the aortic sinus. Immunohistological analysis of lesion morphology included primary antibodies for ICAM-1, VCAM-1, CD4, CD73, and CD11b. Determination of the plaque score by oil red staining revealed enhanced development of atherosclerotic lesions over the entire thoracic aorta after 6 month in the double mutant compared with ApoE−/− (2.5-fold increased, n=8, P |
| Databáze: | OpenAIRE |
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