Determination of Deoxynivalenol-3-Glucoside in Cereals by Hydrophilic Interaction Chromatography with Ultraviolet Detection
| Autor: | Dan Yang, Zhiming Geng, Fang Liu, Miaoping Zhou, Yongzhi Zhu, Muhan Zhang, Pingping Zhang, Daoying Wang |
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| Rok vydání: | 2013 |
| Předmět: |
Detection limit
Chromatography Calibration curve Chemistry Hydrophilic interaction chromatography Analytical chemistry medicine.disease_cause Applied Microbiology and Biotechnology Analytical Chemistry Chromatographic separation Deoxynivalenol-3-glucoside medicine Uv detection Safety Risk Reliability and Quality Safety Research Ultraviolet Food Science |
| Zdroj: | Food Analytical Methods. 7:1139-1146 |
| ISSN: | 1936-976X 1936-9751 |
| DOI: | 10.1007/s12161-013-9726-1 |
| Popis: | A new hydrophilic interaction liquid chromatography (HILIC) method was developed for the determination of deoxynivalenol-3-glucoside (D3G) in cereals. D3G was extracted with water and cleaned with an immunoaffinity (IA) column, followed by chromatographic separation on a Syncronis HILIC column with acetonitrile-water (90:10, v/v) as the mobile phase, and detected at 220 nm by UV detection. The calibration curve was found to be linear in the range of 0.1–2.0 μg/ml. Limit of detection and limit of quantification were 8 and 25 μg/kg, respectively. The method was applied to the determination of D3G in 14 cereal samples, which were analyzed as well as by LC-MS/MS. The results indicate that the method can be considered as an alternative to LC-MS/MS and could be adopted by nonprofessional analytical labs. |
| Databáze: | OpenAIRE |
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