Thioflavin T as luminescence biosensors for nucleic acid study and RNase A activity detection
| Autor: | Changying Yang, Shuang Lu, Han Liu, Jinya Du, Yuzhi Dong, Yang Li, Lingli Gong |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Quenching (fluorescence) Chemistry RNase P 010401 analytical chemistry RNA 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Enzyme Biochemistry Nucleic acid Thioflavin 0210 nano-technology Spectroscopy DNA |
| Zdroj: | Microchemical Journal. 147:842-847 |
| ISSN: | 0026-265X |
| DOI: | 10.1016/j.microc.2019.04.008 |
| Popis: | An applicable RNase assay was constructed based on strong luminescent complex between Thioflavin T (ThT) and RNA. The emission of ThT was more sensitive to RNA than to DNA, and electrostatic interaction played an important role when ThT interacted with RNA. The ThT-RNA fluorescence system displayed high specific quenching for RNase A, which originated from the specific cleavage reaction of RNA by RNase A. The quantitative detection of RNase A was built with a rather low detection limit 2.47 × 10−6 U/mL. Enzymatic kinetics and inhibitory experiments were conducted to demonstrate the utilities of this method in RNase A activity study and screening of enzyme inhibitors in biological samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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