Novel wound dressing based on nanofibrous PHBV-keratin mats
| Autor: | Jung-Chul Kim, Kyoung-Jin Shim, Zhi-Cai Xing, Jiang Yuan, Inn-Kyu Kang, Jia Geng, Jian Shen, Insook Han |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
integumentary system Atomic force microscopy Field emission scanning electron microscopy Composite number Biomedical Engineering Medicine (miscellaneous) macromolecular substances Electrospinning Biomaterials symbols.namesake chemistry Chemical engineering Wound dressing Keratin symbols Wound healing Raman spectroscopy |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 9:1027-1035 |
| ISSN: | 1932-6254 |
| Popis: | Keratin is an important protein used for wound healing and tissue recovery. In this study, keratin was first extracted from raw materials and chemically modified to obtain stable keratin (m-keratin). The raw and m-keratin were examined by Raman spectroscopy. The molecular weight of the m-keratin was analysed by SDS–PAGE. The m-keratin was then blended with poly(hydroxybutylate-co-hydroxyvalerate) (PHBV) and electrospun to afford nanofibrous mats. These mats were characterized by field emission scanning electron microscopy (FE-SEM), electron spectroscopy for chemical analysis (ESCA) and atomic force microscopy (AFM). From the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) data, it was found that introduction of keratin enhanced cell proliferation. From wound-healing test and histological examination results, it was shown that the composite mats accelerated wound recovery remarkably as compared to the PHBV control. It was concluded that PHBV–keratin may be a good candidate as a wound dressing. Copyright © 2012 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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