Oligonucleotide Arrays from Aldehyde-Bearing Glass with Coated Background
| Autor: | Clemens Richert, Niels Griesang, Kendra L. Dombi |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Wax Oligonucleotide Organic Chemistry engineering.material Reductive amination Combinatorial chemistry Aldehyde Catalysis chemistry.chemical_compound Solid-phase synthesis chemistry Coating visual_art visual_art.visual_art_medium engineering Dichloromethane Triethylene glycol |
| Zdroj: | Synthesis. 2002:816-824 |
| ISSN: | 1437-210X 0039-7881 |
| DOI: | 10.1055/s-2002-25758 |
| Popis: | Presented here is a method for preparing small DNA arrays on aldehyde-bearing glass slides. Immobilization involves reductive amination and employs oligonucleotides with 3'-terminal lysine residues, obtained in high yieldfrom solid phase syntheses. Spot patterns are produced by protecting selected areas of the aldehyde slides with wax, coating the free surface with a methyl triethylene glycol derivative, and removing the wax with dichloromethane. The DNA arrays give better signal to noise ratios in hybridization experiments than slides without passified background. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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