Complementation ofMethylobacterium organophilummutants affected in pyrroloquinoline quinone biosynthesis genespqqEandpqqFby clonedEscherichia colichromosomal DNA
| Autor: | Evelyne Turlin, Francis Biville, Francis Gasser |
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| Rok vydání: | 1991 |
| Předmět: |
biology
Methanol dehydrogenase Molecular cloning biology.organism_classification medicine.disease_cause Microbiology Molecular biology Complementation chemistry.chemical_compound Pyrroloquinoline quinone chemistry Biochemistry Glucose dehydrogenase Genetics medicine Methylobacterium Hybrid plasmid Molecular Biology Escherichia coli |
| Zdroj: | FEMS Microbiology Letters. 83:59-63 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1991.tb04389.x |
| Popis: | The hybrid plasmid pBGT3, a derivative of pLA2917 containing a 7.8-kb fragment of Escherichia coli DNA, was found to complement pqqE and pqqF mutants of Methylobacterium organophilum , both impaired in PQQ biosynthesis. The cloned fragment of E. coli DNA did not hybridize with DNA fragments containing pqqE or pqqF previously cloned from M. organophilum . Yet, in M. organophilum mutants, expression of pqqE and pqqF genes from E. coli resulted in a PQQ production estimated at 9–16% of the production observed in M. organophilum wild-type. The growth rate in methanol medium of the complemented M. organophilum mutants was about 60% of that of the wild-type. |
| Databáze: | OpenAIRE |
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