The role of non-stimulatory MHC-peptides in aiding antigen recognition by CD8+ T cells (87.49)
| Autor: | Nicholas R.J. Gascoigne, Pia P. Yachi, Carina Lotz, Jeanette Ampudia |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 178:S137-S137 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | T cells are able to identify minute quantities of antigenic MHC-peptide (MHCp) complexes among a sea of endogenous MHCp presented on an antigen presenting cell. We previously showed that non-stimulatory endogenous peptides can enhance T cell sensitivity to limiting quantities of antigen. Here we extend these findings to different T cell populations, and show that non-stimulatory peptides are most important in antigen recognition by less differentiated cells, such that their ability to enhance antigen recognition is the best in thymocytes, moderate in naïve T cells, and mild in effector T cells. The increased sensitivity to antigen in the presence of non-stimulatory peptides can be seen in the level of effector functions such as cytokine production and killing of target cells, as well as by phenotypic maturation markers, such as CD25, CD69, CD44, CD5 and HSA. The importance of non-stimulatory peptides is inversely proportional to the agonist activity of the stimulatory peptide presented. Each of the endogenous non-stimulatory peptides tested showed similar ability to aid in antigen recognition. This may indicate that the limiting factor in the ability of non-stimulatory MHCp to aid recognition of a class I-restricted antigen is the CD8-MHC interaction, rather than the strength of the TCR-MHCp interaction, which seems to be paramount in class II-restricted systems. Supported by NIH GM065230. |
| Databáze: | OpenAIRE |
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