Direct Cycle Sequencing with ΔTaqr'DNA Polymerase

3'-exonuclease activity. The present study was designed to investigate the use of delta Taq DNA polymerase for direct cycle sequencing. Results show that delta Taq DNA polymerase can be used for direct cycle sequencing of the PCR amplified DNA, either from an asymmetrically amplified template (by PCR), double stranded DNA template, PCR amplified DNA cloned into a plasmid vector or from a single stranded template. The primer to template ratio and number of cycles necessary for best sequence data have been determined. From these results we conclude that delta Taq DNA polymerase is a highly versatile enzyme which can be used for DNA sequence determinations by direct cycle sequencing. -->
ISSN: 1042-5179
DOI: 10.3109/10425179709034047
Přístupová URL adresa: https://explore.openaire.eu/search/publication?articleId=doi_________::5f4e7e0531d2e65d6c891978491c8a4b
https://doi.org/10.3109/10425179709034047
Přírůstkové číslo: edsair.doi...........5f4e7e0531d2e65d6c891978491c8a4b
Autor: Jianguo Fan, Rajinder S. Ranu
Rok vydání: 1997
Předmět:
Zdroj: DNA Sequence. 7:285-288
ISSN: 1042-5179
DOI: 10.3109/10425179709034047
Popis: The delta Taq DNA polymerase is a new, genetically modified version of standard Taq DNA polymerase which lacks the 5'-->3'-exonuclease activity. The present study was designed to investigate the use of delta Taq DNA polymerase for direct cycle sequencing. Results show that delta Taq DNA polymerase can be used for direct cycle sequencing of the PCR amplified DNA, either from an asymmetrically amplified template (by PCR), double stranded DNA template, PCR amplified DNA cloned into a plasmid vector or from a single stranded template. The primer to template ratio and number of cycles necessary for best sequence data have been determined. From these results we conclude that delta Taq DNA polymerase is a highly versatile enzyme which can be used for DNA sequence determinations by direct cycle sequencing.
Databáze: OpenAIRE
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