A glycoprotein isolated from culture filtrates of Ophiostoma ulmi as a mansonone-inducing elicitor on elm callus
| Autor: | D. Yang, M. Hubbes, R.S. Jeng |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Antiserum
chemistry.chemical_classification biology Phytoalexin food and beverages Plant Science biology.organism_classification Elicitor Microbiology chemistry.chemical_compound Tissue culture Biochemistry chemistry Ophiostoma ulmi Galactose Callus Genetics Glycoprotein Ecology Evolution Behavior and Systematics Biotechnology |
| Zdroj: | Mycological Research. 98:295-300 |
| ISSN: | 0953-7562 |
| DOI: | 10.1016/s0953-7562(09)80457-7 |
| Popis: | A 21 kDa glycoprotein, identified as a mansonone-inducing elicitor on elm callus, was purified from culture filtrates of Ophiostoma ulmi using HPLC ion-exchange chromatography. Both aggressive and non-aggressive isolates of O. ulmi yielded this elicitor. The glycoprotein is composed of 19 different amino acids and has a 17–20% carbohydrate content, namely α-glucose, β-glucose, galactose and some other unidentified sugars. Polyclonal antiserum against this elicitor was raised in a New Zealand white rabbit. Western blotting of a fungal filtrate revealed that the antiserum reacted only with the 21 kDa glycoprotein. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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