Gamma-protocadherins are enriched and transported in specialized vesicles associated with the secretory pathway in neurons

Autor:	Greg R. Phillips, Georgia Dolios, Mónica Fernández-Monreal, Hugo H. Hanson, Rong Wang, Robert O'Leary, William G.M. Janssen, Twethida Oung
Rok vydání:	2010
Předmět:	Immunolabeling medicine.anatomical_structure Cadherin General Neuroscience Endoplasmic reticulum Organelle medicine Protocadherin Dendrite Biology COPII Secretory pathway Cell biology
Zdroj:	European Journal of Neuroscience. 32:921-931
ISSN:	0953-816X
Popis:	Gamma protocadherins (Pcdh-γs) resemble classical cadherins and have the potential to engage in cell-cell interactions with homophilic properties. Emerging evidence suggests non-conventional roles for some protocadherins in neural development. We sought to determine whether Pcdh-γ trafficking in neurons is consistent with an intracellular role for these molecules. Here we show that, in contrast to the largely surface localization of classical cadherins, endogenous Pcdh-γs are primarily intracellular in rat neurons in vivo and are equally distributed within organelles of subsynaptic dendritic and axonal compartments. A strikingly higher proportion of Pcdh-γ-containing organelles in synaptic compartments was observed at postnatal day 16. To determine the origin of Pcdh-γ-trafficking organelles, we isolated organelles with Pcdh-γ antibody-coupled magnetic beads from brain organelle suspensions. Vesicles with high levels of COPII and endoplasmic reticulum-Golgi intermediate compartment (ERGIC) components were isolated with the Pcdh-γ antibody but not with the classical cadherin antibody. In cultured hippocampal neurons, Pcdh-γ immunolabeling partially overlapped with calnexin- and COPII-positive puncta in dendrites. Mobile Pcdh-γ-GFP profiles dynamically codistributed with a DsRed construct coupled to ER retention signals by live imaging. Pcdh-γ expression correlated with accumulations of tubulovesicular and ER-like organelles in dendrites. Our results are consistent with the possibility that Pcdh-γs could have a unique function within the secretory pathway in addition to their documented surface roles.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5f43ecf457fa8435e65ce9b3c2167c6b https://doi.org/10.1111/j.1460-9568.2010.07386.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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