CLONAL REGENERATION OF LITCHI (LITCHI CHINENSIS SONN.) VIA SOMATIC EMBRYOGENESIS
| Autor: | Richard E. Litz, Simon H. T. Raharjo |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Acta Horticulturae. :487-490 |
| ISSN: | 2406-6168 0567-7572 |
| DOI: | 10.17660/actahortic.2007.738.61 |
| Popis: | Litchi embryogenic cultures were induced from leaflet explants in recent vegetative flushes of mature phase 'Brewster' trees on modified B5 medium containing 400 mg L -1 glutamine, 200 mg L -1 casein hydrolysate, 30 g L -1 sucrose, 3 g L -1 gellan gum, 4.52 μM 2,4-D and 9.30 μM kinetin. Embryogenic cultures were maintained on semi-solid MS medium supplemented with 4.52 μM 2,4-D and 0.91 μM zeatin at 26±1°C in darkness and with 4-5 week subculture intervals. Maturation of somatic embryos occurred in darkness on semi-solid MS medium without plant growth regulators, and fully enlarged somatic embryos germinated. Plantlets were maintained on 60 ml half-strength MS medium with 200 mg L -1 activated charcoal at 26±1°C under a 16h photoperiod provided by cool white fluorescent lights. Plants were successfully transferred to the greenhouse. |
| Databáze: | OpenAIRE |
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