Rapid profiling of crude scorpion venom using liquid chromatography and its relevance to species identification

Autor:	Rajamohammed Abbas Manthiri, Haseeb A. Khan, A.K. Al Asmari
Rok vydání:	2012
Předmět:	Chromatography biology Leiurus Chemistry Scorpion Venom Fast protein liquid chromatography General Chemistry biology.organism_classification complex mixtures High-performance liquid chromatography Distilled water biology.animal Species identification Protein concentration
Zdroj:	Acta Chromatographica. 24:501-509
ISSN:	2083-5736 1233-2356
DOI:	10.1556/achrom.24.2012.3.12
Popis:	Summary We present the comprehensive chromatographic profiles of three scorpion species, Androctonus crassicauda, Androctonus bicolor, and Leiurus quinquestriatus, commonly inhabited to Middle East regions. Their venoms were milked by electrical stimulation, diluted with distilled water, properly mixed and centrifuged to separate the mucus from venom. The clear supernatant was filtered and the protein concentration was determined. Pre-diluted venoms were chromatographed on FPLC (fast protein liquid chromatography) and RP-HPLC (reversed-phase high-performance liquid chromatography) and the fractions were collected for molecular weight determination. Both techniques have resulted clearly distinguishable chromatographic patterns that can be used for identification of scorpion species and having a quick indication of venom toxicity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5f3c1bfc409ac04295cb890d5eeb7969 https://doi.org/10.1556/achrom.24.2012.3.12 Zobrazit plný text záznamu