Metabonomics study of fresh bruises on an apple using the gas chromatography–mass spectrometry (GC–MS) method

Autor: Wenhao Qin, Ruifeng Li, Zhuobin Guo, Siyu Chen, Shu Zhang, Chizhu Ding, Zuojun Tan
Rok vydání: 2019
Předmět:
Zdroj: European Food Research and Technology. 246:201-212
ISSN: 1438-2385
1438-2377
DOI: 10.1007/s00217-019-03386-x
Popis: Fresh bruise on an apple is a major problem in postharvest, as it can easily be missed at the early stage and then develop into a more serious external bruise as time goes on. In this study, GC–MS was used to investigate the metabolic apple profile change within 24 h after the occurrence of fresh bruising to understand the relationship between quality change and metabolism of fresh bruises on an apple. We identified a total of 36 metabolic components and expounded the early physiological changes and metabolic mechanisms in a freshly bruised apple. Data were analyzed using partial least squares discriminant analysis (PLS-DA) and Euclidean distance and the results showed that 12 h was determined to be the optimal time for loss reduction. We observed that sugars and sugar alditols increased significantly, while amino acids and fatty acid ester decreased dramatically within 24 h. The apple metabolic disorder after fresh bruising is mainly associated with enhancement of respiration, increase of ROS, activation of secondary metabolism, and aggravation of membrane lipid peroxidation. This study highlights the apple metabolic disorder that happens after fresh bruising, providing insights that can further facilitate postharvest apple preservation and reducing the loss of its commercial value.
Databáze: OpenAIRE
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