Electrospun polymer nanofibers as substrate/carrier for engineering of human corneal epithelium
| Autor: | Agate Noer, H. Zell Thime, Aboulghassem Shahdadfar, Leva Momtazi, Ola Nilsen, J.R. Eidet |
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| Rok vydání: | 2016 |
| Předmět: |
technology
industry and agriculture Synthetic membrane macromolecular substances 02 engineering and technology General Medicine Polyethylene glycol 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Cellulose acetate 0104 chemical sciences Ophthalmology chemistry.chemical_compound PLGA Membrane chemistry Tissue engineering Nanofiber PEG ratio Biophysics 0210 nano-technology |
| Zdroj: | Acta Ophthalmologica. 94 |
| ISSN: | 1755-375X |
| Popis: | Purpose Electrospun polymer nanofibers present a structure similar to extracellular matrix (ECM) and support the surrounding cells. The type of polymer used, as well as the polymer blending-ratio, affects wettability. This in turn influences cell growth by altering the types of proteins adsorbed from the solution. Human corneal epithelial cells were grown on electrospun biodegradable and non-biodegradable polymer membranes to evaluate the membranes’ potential within ocular surface tissue engineering. Methods The electrospun polymer membranes consisted of cellulose acetate, poly (lactic-co-glycolic acid) (PLGA), polycaprolactone (PCL), and blends of PCL and polyethylene glycol (PEG). Expression of CDH1 (E-cadherin), ITGA6, ITGB4 (Integrin α6β4) and MKI67 (Ki67) genes were measured in all conditions after 14 days of culture using qRT-PCR. Results Cells seeded on all types of membranes expressed MKI67, which is associated with cell cycle activity and correlates with cell growth. MKI67 expression was high in cells grown on 3:2 blends of PCL and PEG (2.88) and PCL (2.75). This amount was 1.75, 1.00 and 1.59 for cells grown on cellulose acetate, 1:1 blend of PCL and PEG and PLGA respectively. The amount of ITGA6, ITGB4 expression was 1.72 in cells grown on PCL membranes, 1.10 for 3:2 blend of PCL and PEG, 1.05 for cellulose acetate, 1.53 for PLGA and 1.01 for 1:1 blend of PCL and PEG. Only cells grown on membranes containing blends of PCL: PEG showed CDH1 expression, which is critical for cell-cell contact. Conclusions All polymer membranes enabled cellular adhesion and proliferation. PCL:PEG and PCL membranes appear to promote the highest proliferative activity and strongest cell-membrane/cell-cell adhesion. Thus, these might be the most promising substrates for epithelial ocular surface tissue engineering. |
| Databáze: | OpenAIRE |
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