Confocal Microscopy Observation of CHO Cells Cultivated in Presence of Fluorescent – Labelled Rapeseed Peptides

Autor:	D. Dumas, A. Kastner, R. Vanderesse, A. Marc, Céline Frochot
Rok vydání:	2010
Předmět:	Chinese hamster ovary cell Biology Fluorescence law.invention Cell membrane medicine.anatomical_structure Biochemistry Cytoplasm Confocal microscopy law medicine Cytotoxic T cell Receptor Incubation
Zdroj:	Cells and Culture ISBN: 9789048134182
Popis:	Plant peptides of a rapeseed peptone were labelled with a coumarin fluorescent probe. The free-probe does not incorporate cell cytoplasm and is not cytotoxic at a concentration below 10 μM. Observations by Confocal Microscopy of CHO cells incubated in presence of labelled peptides indicate no fixation of the peptides on cell membrane receptors, but their incorporation inside cell cytoplasm. This integration seems to be time-dependant while the fluorescence intensity reaches saturation after more than 12 hours of incubation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5f1808194585e2b8fd8fe7881d3df142 https://doi.org/10.1007/978-90-481-3419-9_37 Zobrazit plný text záznamu