Cytogenetic characterization and nuclear DNA content of three North African endemic Centaurea species
| Autor: | N. Khalfallah, K. Baziz, M. Benamara-Bellagha, Fatima Pustahija, S. Siljak-Yakovlev |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics biology medicine.diagnostic_test Heterochromatin Locus (genetics) Karyotype Plant Science Ribosomal RNA biology.organism_classification 010603 evolutionary biology 01 natural sciences Nuclear DNA 03 medical and health sciences 030104 developmental biology Centaurea medicine Genome size Ecology Evolution Behavior and Systematics Fluorescence in situ hybridization |
| Zdroj: | Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 150:501-511 |
| ISSN: | 1724-5575 1126-3504 |
| Popis: | Three endemic Centaurea species from North Africa are investigated for the first time by chromomycin fluorochrome banding for GC-rich DNA distribution, fluorescence in situ hybridization for physical mapping of rRNA genes, and flow cytometry for genome-size assessment. Investigated species belong to three different sections and possess three basic chromosome numbers: C. tougourensis subsp. tougourensis 2n = 4x = 36 (x = 9), C. musimonum 2n = 2x = 20 (x = 10), and C. maroccana 2n = 2x = 24 (x = 12). The number and distribution of chromomycin positive bands (CMA+) and 18S-5.8S-26S (35S) rDNA loci were different among investigated species and ranged from 6 to 80 chromomycin bands and from 2 to 6 35S rDNA loci. The three species have just one 5S rDNA locus at intercalary position on a separate chromosome pairs, except in the case of C. musimonum in which both rDNA loci were localized on the same chromosome. All rDNA loci were co-localized with CMA+ bands, except three 35S in C. musimonum. Genome size ranged f... |
| Databáze: | OpenAIRE |
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