Active transforming growth factor-β in human melanoma cell lines: No evidence for plasmin-related activation of latent TGF-β
| Autor: | Jozef Bizik, Grófová M, Diana Felnerova, Antti Vaheri |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
TGF alpha biology Plasmin Melanoma Growth factor medicine.medical_treatment Cell Biology Transforming growth factor beta medicine.disease Biochemistry Endocrinology Cell culture Internal medicine TGF beta signaling pathway Cancer research medicine biology.protein Molecular Biology Plasminogen activator medicine.drug |
| Zdroj: | Journal of Cellular Biochemistry. 62:113-122 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/(sici)1097-4644(199607)62:1<113::aid-jcb12>3.0.co;2-o |
| Popis: | Cultured human melanoma cells were found to secrete TGF-beta mostly in latent biologically inactive form but in addition five of six melanoma cell lines studied produced in conditioned culture medium active TGF-beta in the range from 370 to 610 pg per 10(6) cells per 24 h. A distinct characteristic of these melanoma cell lines is that they form active surface-bound plasmin by the activation of plasminogen with surface-bound tissue-type plasminogen activator. The present study was performed to assess the role of plasmin in the process of latent TGF-beta activation in the melanoma cell lines. No direct correlation was found between cell-associated plasmin activity and the amount of active TGF-beta present in the conditioned medium of individual cell lines. The melanoma cell lines exhibited diverse responses to exogenous active TGF-beta 1; three cell lines were growth-stimulated, two were growth-inhibited, and one had a very low sensitivity to the growth factor. The active TGF-beta produced by the melanoma cells was found to inhibit the natural killer cell function of peripheral blood lymphocytes, suggesting that it may have an immunosuppressive effect and a role in the development of melanomas. |
| Databáze: | OpenAIRE |
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