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Analyses for TNT and nine potential metabolites (TNT-related compounds) were made in deer, rabbit, and quail tissues (muscle and liver) taken from the Alabama Army Ammunition Plant (AAAP), Childersburg, Alabama. The listed TNT-related compounds are 2,4,6- trinitrotoluene (parent compound); 2,4-diamino-6-nitrotoluene; 2,6-diamino-4-nitrotoluene; 2-amino-4,6-dinitrotoluene; 4-amino-2,6- dinitrotoluene; 2,4,6-trinitrobenzyl alcohol; 2,4,6-trinitrobenzoic acid; 1,3,5-trinitrobenzene; 4-hydroxylamino-2,6-dinitrotoluene; and 2,6,2',6'-tetranitro-4,4'-azoxytoluene. The procedure for extraction of these compounds from animal tissue required homogenization in acetonitrile, and subsequent partitioning into chloroform. Quantitative determination of extracted compounds was obtained by chromatographic separation on a mixed-mode HPLC column in which the phase bonded to the silica surface contained both a C18 (reversed-phase function) and a secondary amine (anion exchange function) incorporated into a single ligand. A ternary mobile phase gradient containing pH 5.1 phosphate buffer, methanol, and acetonitrile was used in separation. An experimental verification of the metabolism of TNT and the detection (or absence) of the selected metabolites was performed in mice subacutely dosed with 100 milligrams per kilogram of ({sup 14}C)-TNT. These studies show that the TNT-related compounds of concern do accumulate in muscle and liver tissue of the mouse under the experimental conditions imposed, but at concentrations below the 1.2 ppM level. However, products other than TNT and freemore » metabolites may be accumulating since some ({sup 14}C) was found to be nonextractable. 13 refs., 5 figs., 6 tabs.« less |
