JAK-STAT pathway activation in response to spinal cord injury in regenerative and non-regenerative stages ofXenopus laevis
| Autor: | Juan Larraín, Victor S. Tapia, Mauricio Herrera-Rojas |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Ependymal Cell biology Regeneration (biology) Xenopus JAK-STAT signaling pathway General Medicine Anatomy biology.organism_classification Spinal cord medicine.disease Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure SOX2 medicine Spinal cord injury 030217 neurology & neurosurgery Spinal Cord Regeneration |
| Zdroj: | Regeneration. 4:21-35 |
| ISSN: | 2052-4412 |
| DOI: | 10.1002/reg2.74 |
| Popis: | Xenopus laevis tadpoles can regenerate the spinal cord after injury but this capability is lost during metamorphosis. Comparative studies between pre-metamorphic and metamorphic Xenopus stages can aid towards understanding the molecular mechanisms of spinal cord regeneration. Analysis of a previous transcriptome-wide study suggests that, in response to injury, the JAK-STAT pathway is differentially activated in regenerative and non-regenerative stages. We characterized the activation of the JAK-STAT pathway and found that regenerative tadpoles have an early and transient activation. In contrast, the non-regenerative stages have a delayed and sustained activation of the pathway. We found that STAT3 is activated in response to injury mainly in Sox2/3+ ependymal cells, motoneurons and sensory neurons. Finally, to study the role of temporal activation we generated a transgenic line to express a constitutively active version of STAT3. The sustained activation of the JAK-STAT pathway in regenerative tadpoles reduced the expression of pro-neurogenic genes normally upregulated in response to spinal cord injury, suggesting that activation of the JAK-STAT pathway modulates the fate of neural progenitors. |
| Databáze: | OpenAIRE |
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